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Alice Cheung



LGRT 1226
(413) 545-4027


Molecular and cell biology of male-female interaction and polarized cell growth in plant reproduction

Background and Training

PhD: Yale University
Postdoctoral training: Harvard University

Research Summary

The two current major research areas in our laboratory share the common focus of dissecting the signal transduction pathways that mediate plant cell responses to external growth and developmental stimuli. 

One area of our work is understanding the signaling of directional pollen tube growth in plant sexual reproduction. Pollen tubes grow in a polar fashion to transport the sperm cells over long distances within the female tissues. The pollen tube growth process is driven by the pollen cytoplasm but is modulated by signals that originate from the female tissues to nourish and guide them to the egg cell. Our efforts are to identify female signal molecules, their receptors on the pollen surface, and signalling molecules that regulate the pollen cellular machinery for growth.

Among the molecules we are working with are female proteins that stimulate and attract pollen tube growth, pollen surface receptor kinases, pollen cell membrane associated Rac-like G-proteins, their downstream effectors in the form of kinases, the actin regulatory protein cofilin and the actin cytoskeleton (the underlying cellular system that drives the pollen tube growth process), and another family of G-proteins known as Rabs that regulate vesicular transport activities, which is essential for pollen tube elongation. Our ultimate goal is to connect these molecules in a signaling pathway that links the female stimuli to the pollen cellular response.

The second area of our work is understanding the signaling of plant growth regulators. Our current focus is the signaling of auxin-mediated gene expression. Despite its importance and extensive understanding of auxin actions, little is known about how this hormone is perceived at the cell surface and transduced to the cytosol to initiate downstream signaling pathways. Our efforts thus far led to the identification of cell surface associated Rac-like G-proteins as mediators of the auxin signal to auxin-responsive genes. Our ultimate goal is to dissect the cross talk and divergence among various signaling pathways that together produce at least a subset of the highly diverse auxin response.


Cheung, A.Y., Wu, H-M. 2015. Serks: Putting plant mouths in their right place. Curr. Biol. In press

Li, C., Yeh, F-L., Cheung, A.Y., Duan, Qh, Kita, D., Liu, M-C., Maman, J., Luu, E.J., Wu, B.W., Gates, L., Jalal, M., Kwong, A., Carpenter, H., Wu, H-M. 2015. Glycosylphosphatidylinositol-anchored proteins act as chaperones and co-receptors for FERONIA receptor kinase signaling in Arabidopsis. eLife, 4:e06587. DOI: [PubMed]

Cheung, A.Y., Li, C., Zou, Y.J., Wu, H.M. 2014. Glycosylphosphatidylinositol anchoring: control through modification. Plant Physiol. 166, 748-750. doi: http:/​/​dx.​doi.​org/​10.​1104/​pp.​114.​246926 [PubMed]

Huang, J.B., Liu, H., Chen, M., Li, X., Wang, M., Yang, Y., Wang, C., Huang, J., Liu, G., Xu, J., Cheung, A.Y., Tao, L.Z. 2014. ROP3 GTPase contributes to polar auxin transport and auxin responses and is important for embryogenesis and seedling growth in Arabidopsis. Plant Cell 26, 3501-3518. doi: http:/​/​dx.​doi.​org/​10.​1105/​tpc.​114.​127902 [PubMed]

Duan, Q, Kita D., Johnson, E.A., Aggarwal, M., Gates, L.*, Wu, H-M., Cheung, A.Y. 2014. Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis. Nature Commun. 5, 3129.  Doi:10.1038/ncomms4129. [PubMed]

Hernandez, A., Quito, C., Johnson, E.A., Wu, H-M., Cheung, A.Y., Cardenas, L. 2013. Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: a method with virtually unlimited potential in plant biology. Methods in Enzymology 527, 275-290. doi: 10.1016/B978-0-12-405882-8.00015-5 [PubMed]

Nibau, C., Tao, L-z.,Levasseur, K., Wu, H-M., Cheung, A.Y.  2013. The Arabidopsis small GTPase AtRac7/ROP9 is a modulator of auxin and ABA signaling. J. Exp. Bot., 64, 3425-3437. Doi:10.1093/jxb/ert179 [PubMed]

Chen, C. Y., Zheng, W-G., Wu, H.-M., Cheung, A.Y. 2013. Pollen germination activates theapical membrane located RAC/ROP GTPase switch. Molecular Plant, 6, 1358-1361. doi: 10.1093/mp/sst074. (Cover)

Wu, H-M, Cheung, A.Y. 2011. THESEUS and relatives: cell wall-sensing receptor-like kinases. Curr. Opin. Plant Biol.  14, 632-641. [PubMed]

Chen, M.,  Liu,H., Kong, X.,  Zhang,N., Yang, Y.,Li, R., Jianbin Yue, J., Chen, D., Tian, R., Li, C., Cheung, A.Y., Tao, L-z.  2011.  The RopGEF7 is required to control Plethora (PLT)-dependent maintenance of root stem cell niche in Arabidopsis.  Plant Cell 23, 2880-2894.

Wu, H-M., Hazak, O., Cheung, AY., Yalovsky, S. RAC/ROP GTPases and Auxin Signaling. 2011. The Plant Cell. [PubMed]

Lu, Y., Chanroj, S., Zulkifli, L., Johnson, M., Uozumi, N., Cheung, A.Y., Sze, H. Pollen Tubes Lacking a Pair of K+ Transporters Fail to Target Ovules in Arabidopsis. 2011. The Plant Cell, 23: 81-93. [PubMed]

Nibau C., Di Stilio V.S., Wu, H-M., Cheung A.Y. Arabidopsis and Tobacco superman regulate hormone signalling and mediate cell proliferation and differentiation. 2010. J. Exp. Bot. 62, 949-961. [PubMed]

Duan Q., Kita D., Li C., Cheung A.Y. and Wu H-M. FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development. Proc. Nat'l Acad. Sci. USA 2010, 107: 17821-17826. [PubMed]

Cheung, A.Y, Boavida,L., Aggarwal, M., Wu, H-M, Feijo J. The Pollen Tube Journey in the Pistil and Imaging the In Vivo process by two-Photon Microscopy. J. Exp. Bot. 2010, 61:1907-1915. [PubMed]

Cheung AY, Q Duan, S Santos Costad, BHJ de Graaf, VS Di Stilio, J Feijod, and H-M Wu. The Dynamic Pollen Tube Cytoskeleton: Live Ce l Studies Using Actin-Binding and Microtubule-Binding Reporter Proteins. 2008. Molecular Plant. [PubMed]

Nibau, C., Wu, H-M., Cheung, A. (2006) RAC/ROP GTPases: 'hubs' for signal integration and diversification in plants. Trends Plant Sci. June. [PubMed]

Li-zhen Tao, Alice Y. Cheung, Candida Nibau, and Hen-ming Wu . (2005). RAC GTPases in Tobacco and Arabidopsis Mediate Auxin-Induced Formation of Proteolytically Active Nuclear Protein Bodies That Contain AUX/IAA Proteins. Plant Cell 17: 2369-2383. [PubMed]

Barend H.J. de Graaf, Alice Y. Cheung, Tatyana Andreyeva, Kathryn Levasseur, Marcia Kieliszewski, and Hen-ming Wu (2005). Rab11 GTPase-Regulated Membrane Trafficking Is Crucial for Tip-Focused Pollen Tube Growth in Tobacco. Plant Cell 17: 2564-2579.[PubMed]